Specific Aims
To provide technical support and consultation in DNA sequencing, quantitative gene expression, molecular cloning, DNA/RNA isolation, and DNA mutation analysis, among others.
To further develop the Genotyping Center by expansion of the current facilities and access to expertise in genotyping and genetic linkage.
To provide access to thermocyclers, automated sequencers, PAGE electrophoresis systems, whole-genome microsatellite markers, and real-time RT-PCR.
To encourage the development of new projects and collaborations.
EDUCATION
University of Puerto Rico Mayagüez, PR. BA, Industrial Microbiology, 1988.
Rutgers University/Robert Wood Johnson Medical School. MS, Molecular Genetics and Microbiology, 1992.Rutgers University/Robert Wood Johnson Medical School PhD, Molecular Genetics and Microbiology, 1994.
Idhaliz Flores, PhD
Description of Facilities
The MBCL is currently assisting in several projects that study the molecular pathogenesis of several diseases that affect our (the Puerto Rican) population; these include diabetes, cancer albinism and edometriosis.
In addition, we are conducting three genetic linkage studies on Bardet-Biedl, Jarcho-Levin, and endometriosis.
Our facilities were instrumental in the cloning and characterization of two novel dust mite genes that are homologous to allergens known to exacerbate asthma symptoms. In two other collaborations, we are using DHPLC (WAVE System, Transgenomic Inc.) to identify mutations associated with early-onset basal cell carcinoma and colon carcinoma arising in IBD.
